| # | Title | Artist | Rating | Length |
|---|
| 1 | Parchman Farm Blues | Bukka White | | 2:42 |
| 2 | Roll and Tumble Blues | Hambone Willie Newbern | | 3:03 |
| 3 | God Moves on the Water | Blind Willie Johnson | | 3:02 |
| 4 | Graveyard Dream Blues | Bessie Smith | 5 | 2:45 |
| 5 | Mama, 'Tain't Long Fo'Day | Blind Willie McTell | | 2:58 |
| 6 | Arwhoolie (Cornfield Holler) | Thomas J. Marshall | | 0:44 |
| 7 | Screamin' and Hollerin' the Blues | Charley Patton | | 3:09 |
| 8 | Future Blues | Willie Brown | 5 | 3:01 |
| 9 | Preaching the Blues, Part II | Son House | | 2:54 |
| 10 | Bye Bye Blues | Tommy Johnson | 5 | 3:04 |
| 11 | Black Cat Blues (take 1) | Memphis Minnie | | 2:57 |
| 12 | 32-20 Blues | Roosevelt Sykes | | 3:09 |
| 13 | Hard Time Killin' Floor Blues | Skip James | | 2:50 |
| 14 | Up Country Blues | De Ford Bailey | | 3:15 |
| 15 | Old Original Kokomo Blues | Kokomo Arnold | | 2:53 |
| 16 | Easy Rider Blues | Blind Lemon Jefferson | | 2:57 |
| 17 | C C Rider | Lead Belly | | 4:09 |
| 18 | Toad Frog Blues | Ma Rainey | | 3:04 |
| 19 | The Jazz Fiddler | Mississippi Sheiks | | 3:13 |
| 20 | She's Making Whoopee in Hell Tonight | Lonnie Johnson | | 3:12 |
| 21 | Noah | The Golden Gate Quartet | | 2:37 |
| 22 | I'm Throwin' Up My Hands (Ain't Gonna Work Here No More) | Reverend Gary Davis | | 2:50 |
| 23 | I Looked Down the Lane (And I Wondered) | Sister Rosetta Tharpe | | 2:54 |
| 24 | Falling Down Blues | Furry Lewis | 5 | 2:52 |
| 25 | Mistreatin' Mama | Big Bill Broonzy | | 3:00 |