| # | Title | Artist | Rating | Length |
|---|
| 1 | Cotton Eyed Joe | Stanley Brothers | | 1:13 |
| 2 | New Mule Skinner Blues | Bill Monroe | | 2:31 |
| 3 | Happy Valley Special | Bailey Brothers | | 2:21 |
| 4 | Duelin’ Banjos | Don Reno & Arthur Smith | | 1:57 |
| 5 | I’m Going to Make Heaven My Home | Flatt & Scruggs | | 2:38 |
| 6 | In the Hills of Roane County | The Blue Sky Boys | | 3:16 |
| 7 | Go Along Mule | Uncle Dave Macon | | 3:04 |
| 8 | Orange Blossom Special | The Rouse Brothers | | 2:58 |
| 9 | She’s Just a Cute Thing | Jimmy Martin & The Osborne Brothers | | 1:56 |
| 10 | Air Mail Special | Jim and Jesse | | 2:06 |
| 11 | Don’t Get Above Your Raisin’ | Flatt & Scruggs | | 2:42 |
| 12 | I’m Going Back to Old Kentucky | Bill Monroe | | 2:56 |
| 13 | Banjo Pickin’ Girl | The Coon Creek Girls | | 2:48 |
| 14 | Mountain Dew | Grandpa Jones | | 2:47 |
| 15 | I’m Gone Long Gone | Reno and Smiley | | 2:20 |
| 16 | The Great Speckled Bird | Charlie Monroe's Boys | | 2:13 |
| 17 | Roll in My Sweet Baby’s Arms | Bill & Charlie Monroe | | 2:29 |
| 18 | Doin’ My Time | Jimmie Skinner | | 2:34 |
| 19 | Poor Ellen Smith | Molly O’Day | | 2:41 |
| 20 | Will the Circle Be Unbroken | Brownsferry Four | | 3:00 |